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马铃薯TCP家族转录因子鉴定与表达模式分析

Identification and Expression Pattern Analysis of TCP Family Transcription Factors in Potato

  • 摘要: TCP (teosinte branched1/cycloidea/proliferating cell factor)是植物特有的一类转录因子,参与植物的细胞信号传导,并在植物生长发育过程中发挥重要的调控作用。马铃薯(Solanum tuberosum)是世界四大粮食作物之一,为了探索TCP转录因子在马铃薯生长发育中的作用,本研究通过生物信息学方法,对马铃薯TCP转录因子进行了全基因组检索,并借助表达数据库对这些TCP转录因子进行了表达模式分析。本研究从马铃薯基因组数据库中检索出了27个StTCPs基因,长度为657~1 347 bp,编码氨基酸201~534个。马铃薯TCP蛋白均为亲水性蛋白,分子量为21 143.87~59 686.47 Da,等电点在5.22~10.08;除1个定位于细胞核和叶绿体外,其余26个TCP均定位于细胞核。保守基序分析结果显示,马铃薯TCP家族成员的保守基序构成不同,但均具有bHLH保守结构域。同源性分析结果表明,马铃薯TCP与拟南芥(Arabidopsis thaliana)、烟草(Nicotiana tabacum)TCP的同源性较高,而与水稻(Oryza sativa) TCP的同源性较低。组织表达模式分析结果显示,马铃薯TCP基因的表达具有组织特异性。其中,StTCP16和StTCP19基因在马铃薯茎及幼小块茎中表达水平较高。响应诱导表达模式分析表明,StTCP13、StTCP22、StTCP24基因的表达受IAA等激素的强烈诱导;在盐和甘露醇胁迫下,StTCP13基因的表达量则明显降低。本研究结果为马铃薯TCP转录因子的生物学功能研究提供了基础。

     

    Abstract: TCP(teosinte branched1/cycloidea/proliferating cell factor) is a kind of transcription factors peculiar to plants, which participates in the plant cell signal transmission and plays an important regulatory role in the process of plant growth and development. Potato(Solanum tuberosum) is one of the four major food crops in the world.In order to explore the role of TCP transcription factors in potato growth and development, this study carried out a whole-genome retrieval of potato TCP transcription factors by means of bioinformatics, and analyzed the expression patterns of these TCP transcription factors by means of the expression database. 27 StTCPs geneswith657~1 347 bp in length and encoding 201~534 amino acids were retrieved from the potato genome databa se. All potato TCP proteins are hydrophile proteins with molecular weights ranging from 21 143.87~59 686.47 Da and isoelectric points ranging from 5.22~10.08. Except one TCP was located in nucleus and chloroplast, the other 26 TCP were located in nucleus. The results of conservative motif analysis showed that the conservative motif composition of the members of the potato TCP family is different, but all of them have bHLH conservative domain.The homology analysis results showed t hat potato TCP had higher homology with Arabidopsis thaliana and tobacco TCP, but lower homology with rice TCP. Tissue expression pattern analysis showed that the expression of potato TCP gene was tissue specific. Among them, StTCP16 and StTCP19 genes were highly expressed in potato stems and young tubers. The expression pattern analysis of response induction showed that the expression of StTCP13, StTCP22 and StTCP24 genes was strongly induced by IAA and other hormones. Under salt and mannitol stress, the expression of StTCP13 gene was significantly decreased. The results of this study provided the basis for the biological functions research of TCP transcription factor in potato.

     

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