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基于网络药理学探讨野菊花治疗鼻咽癌的可能作用机制

The Possible Mechanism o f Treating Nasopharyngeal Carcinoma with Flos Chrysanthemi Indici Based on Network Pharmacology

  • 摘要: 野菊花(Flos chrysanthemi indici)是传统中药材,其提取物能有效抑制多种肿瘤细胞。鼻咽癌(nasopharyngeal carcinoma, NPC)是中国发病率较高的头颈部恶性肿瘤,目前野菊花对其是否有疗效尚不清楚。本研究拟通过网络药理学探讨野菊花治疗鼻咽癌的作用靶点及作用机制。运用TCMSP数据库以药物口服生物利用度(OB≥30%)及类药性(DL≥0.18)为依据检索野菊花活性化合物和作用靶点,通过GeneCards数据库搜索NPC相关疾病靶点,使用Cytoscape 3.6.1软件构建野菊花活性化合物-鼻咽癌疾病靶点互作网络图,并运用DAVID数据库进行GO功能注释及KEGG通路富集分析。结果显示出野菊花治疗鼻咽癌的活性化合物有6个,潜在靶点有51个,活性化合物-关键靶点-通路网络图显示关键靶点主要包括:TP53、JUN、CASP3、CASP9、EGFR、PTGS2、BCL2L1、CASP8等。GO功能注释获得分子功能条目30个、生物过程条目122个和细胞组成条目18个,KEGG通路富集得到20条相关通路,涉及PI3K-Akt信号、癌症信号、细胞凋亡和EB病毒感染等信号通路。上述结果表明,野菊花发挥治疗鼻咽癌的作用主要是通过调节TP53、JUN、EGFR、PTGS2等靶点及相关信号通路,进而发挥对鼻咽癌的治疗作用。

     

    Abstract: Flos chrysanthemi indici is a traditional Chinese medicinal material, and its extract can effectively inhibit a variety of tumor cells. Nasopharyngeal carcinoma(NPC) is a head and neck malignant tumor with high incidence in China, but it is not clear whether Flos chrysanthemi indici has curative effect on. To explore the target and mechanism of wild chrysanthemum in the treatment of nasopharyngeal carcinoma by network pharmacology, the active components and action targets of Flos chrysanthemi indici were searched by TCMSP database based on oral bioavailability(OB≥30%) and drug-like drugs(DL≥0.18). The NPC-related disease targets were searched by GeneCards database. The Flos chrysanthemi indici active component-nasopharyngeal carcinoma disease target network map was constructed by Cytoscape 3.6.1 software, and GO functional annotations and KEGG pathway enrichment analysis were carried out by using DAVID database. The results showed that six active components and51 potential targets of Flos chrysanthemi indici in the treatment of nasopharyngeal carcinoma were obtained. The active component-key target-pathway network map showed that the key targets included TP53, JUN, CASP3,CASP9, EGFR, PTGS2, BCL2 L1, CASP8 and other targets. 30 molecular functional items, 122 biological process items and 18 cell composition items were obtained by GO functional annotations, and 20 related pathways were obtained by KEGG pathway enrichment, involving PI3 K-Akt signal pathway, cancer signal pathway, apoptosis pathway and EB virus infection pathway and other signal pathways. The above results indicate Flos chrysanthemi indici in the treatment of nasopharyngeal carcinoma is mainly through TP53, JUN, EGFR, PTGS2 and other targets and related signal pathways, and then play a role in the treatment of nasopharyngeal carcinoma.

     

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