Abstract: The functional role of long-chain non-coding RNA LINC00324 in the genesis and development of acute myeloid leukemia（AML） remains unknown. To investigated the relationship between genome-wide expression level of LINC00324 and karyotype and prognosis in AML patients, firstly, real-time fluorescence quantitative PCR was carried out to detect the expression level of LINC00324 in 46 patients, and its relationship with chromosome karyotype and gene changes was analyzed. Secondly, cBioPortal database was utilized to analyze the relationship between LINC00324 expression level and clinical indicators in 173 AML patients. Experimental results exhibited that the expression level of LINC00324 was significantly reduced（P＜0.001） in AML patients, particularly in the poor prognosis group（P ＜0.05）; Data obtained from cBioPortal database also indicated that the level of LINC-00324 was significantly reduced in both the medium prognosis group and the poor prognosis group（P＜0.01）, and the poor prognosis group LINC00324 level was lower（P＜0.05）. Furthermore, in the group with poor prognosis, LINC00324 expression level in patients with monosomal karyotype was lower than that of patients with non-monosomal karyotype（P＜0.05）. Moreover, LINC00324 expression level was significantly down-regulated in FLT3-ITD positive patients, M0, M1, and M2 patients（P＜0.05）. Additionally, data also showed LINC00324 levels were negatively correlated with WT1 expression, C-KIT expression, and the number of bone marrow blasts（P＜0.001）. The results indicate that LINC00324 may be involved in the occurrence and development of AML, and is expected to be a potential indicator for the prognostic evaluation of AML patients.