Abstract: To study the effect of Gan-song Yin（HGY） on the expression of FN, Col1α1 and Col4α1 genes and proteins in the rats model of diabetic kidneys disease（DKD）. Rat models of diabetic kidney disease were prepared and divided into normal control group（Normal）, DKD model group（DKD）, gliquidone control group（DKD +Gli,10 mg/kg）, HGY high dose group（DKD+HGY, 10 g/kg） and HGY low-dose group（DKD+HGY, 5 g/kg）. Western Blot and immunohistochemical methods were used to detect the protein expression levels of FN, ColⅠ, ColⅣ in DKD rat, and real-time fluorescence quantitative PCR method was used to detect the gene expression levels of FN,Col1α1 and Col4α1 genes in DKD rat. Compared with DKD, HGY can significantly reduce the protein levels of FN,ColⅠ, ColⅣ and the gene expression levels of FN, Col1α1, Col4α1 in DKD rat（P＜0.05）, and HGY is basically similar to gliquinone in regulation. HGY can down-regulate the protein levels of FN, ColⅠ, ColⅣ and the gene expression levels of FN, Col1α1, Col4α1 in early DKD rats, which may delay the development of DKD by improving extracellular matrix（ECM） accumulation.