Abstract: To study the genetic characteristics of yak acid ribosomal protein（P1） and its expression in various tissues, the yak RPLP1 gene was amplified by gene cloning and RT-PCR and the full-length sequence was obtained and analyzed by biological software. The total RNA of heart, liver, lung, spleen, kidney and muscle tissues of Datong yak in Qinghai was extracted. The relative expression of RPLP1 gene in different tissues was detected by RT-qPCR. The open reading frame of RPLP1 gene was 345 bp in length and encoded 114 amino acids. The theoretical relative molecular mass was 2.4 kD, and the isoelectric point pI value was about 5.75. The physicochemic alproperties of RPLP1 protein were stable and the whole peptide chain was hydrophobic. No signal peptide, no transmembrane region, mainly plays a role in ribosomes. The secondary structure of this protein is mainly composed of α helix（51.75%）, extended chain（9.65%）, random coil（35.09%）; homology comparison results showed that the yak had the closest relationship with the common cattle; RT-PCR results showed that the RPLP1 gene was expressed in different degrees in seven tissues. The relative expression in muscle tissue was significantly higher than that in other tissues（P＜0.01）. This study laid a foundation for the functional study of the subsequent yak RPLP1 gene.