美国红枫ArARF18基因的克隆及表达分析

李建一; 王志昆; 韩璇; 朱文鹏; 崔金腾; 赵和文

doi:10.13417/j.gab.040.003189

美国红枫ArARF18基因的克隆及表达分析

李建一¹,
王志昆¹,
韩璇¹,
朱文鹏¹,
崔金腾^1,2,3, ,,
赵和文^1, ,

1. 北京农学院园林学院;
2. 城乡生态环境北京实验室;
3. 北京市乡村景观规划设计工程技术研究中心

基金项目:

城乡生态环境北京实验室项目(PXM2019_014207_000012)

2019年北京高等学校高水平人才交叉培养实培计划深化项目(PXM2020_014207_000009)

学位与研究生教育改革与发展项目(2018YJS047)

科技创新服务能力建设-基本科研业务费(追加)(PXM2018_014207_000063)共同资助

详细信息

通讯作者:
崔金腾,cuijinteng@163.com

赵和文,zhaohewen6@sina.com

中图分类号: S687
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出版历程
- 刊出日期: 2021-10-24

Cloning and Expression Analysis of ArARF18 Gene in Acer rubrum L.

1. College of Landscape Architecture, Beijing University of Agriculture;
2. Beijing Laboratory of Urban and Rural Ecological Environment;
3. Beijing Engineering Research Center of Rural Landscape Planning and Design

摘要

摘要: 本研究采用不同浓度IBA处理红花槭(Acer rubrum L.)插条进行嫩枝扦插，通过根系表型性状及生化指标进行了分析，300 mg/LIBA处理组的红花槭插条生根效果最好，一级根数量、二级根数量、平均根尖总数及平均根长均显著优于其他处理。300 mg/LIBA处理组的IAAO和PPO活性较高，POD活性较低，以上均有利于根系生长。因此，IBA 300 mg/L浓度较适宜红花槭嫩枝扦插生根。对红花槭ArARF18基因进行了克隆和表达分析，ArARF18基因ORF长度为2 112 bp，编码703个氨基酸。300 mg/LIBA处理组ArARF18基因表达量显著高于CK组。运用生物信息学方法发现ArARF18氨基酸序列包含B3,Auxin＿resp family和AUX＿IAA family三个ARF家族结构域。本研究可为深入探究植物生长素信号转导通路相关转录因子的研究提供理论和实验依据。
- 红花槭 /
- 生长素响应因子 /
- ArARF18 /
- 生物信息学
Abstract: This research adopts the different concentration of IBA treatment the Acer rubrum L. to twig cutting,cutting through the root phenotypic traits and biochemical index was analyzed, and 300 mg/L IBA treatment group of A. rubrum cuttings rooting effect is best, primary root number, secondary average root number, root and total average root length were significantly higher than other processing. The 300 mg/L IBA group showed higher activity of IAAO and PPO and lower activity of POD, all of which were favorable for root growth. Thus, the concentration of 300 mg/L IBA was more suitable for cutting rooting ofA. rubrum. ArARF18 gene was cloned and expressed in Acer rubrum. The ORF length of ArARF18 gene was 2 112 bp, encoding 703 amino acids. ArARF18gene expression was significantly higher in the 300 mg/L IBA group than in the CK group. Three ARF family domains, B3, Auxin＿resp family and AUX＿IAA family, were found by bioinformatics. It provides theoretical and experimental basis for the in-depth study of auxin signal transduction pathway related transcription factors.
- Acer rubrum L. /
- Auxin response factor /
- ArARF18 /
- Bioinformatics

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