miR-1和miR-206在不同饲养方式AA肉仔鸡肌肉组织中的表达分析

吴秋雪; 张伟伟; 常鑫; 张磊; 邵淑丽; 孙婴宁

doi:10.13417/j.gab.041.000034

miR-1和miR-206在不同饲养方式AA肉仔鸡肌肉组织中的表达分析

吴秋雪¹,
张伟伟^1,2, ,,
常鑫¹,
张磊¹,
邵淑丽^1,2,
孙婴宁^1,2

1. 齐齐哈尔大学生命科学与农林学院;
2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室

基金项目:

国家青年科学基金项目(31801148)

黑龙江省自然科学基金项目(C2016057)

黑龙江省教育厅基本业务专项(135209260)

黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201874)共同资助

详细信息

通讯作者:
张伟伟,zww121@163.com

中图分类号: S831.2
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出版历程
- 刊出日期: 2022-01-24

Expression Analysis of miR-1 and miR-206 in Muscle Tissues of AA Broilers with Different Feeding Methods

1. College of Life Science and Agroforestry, Qiqihar University;
2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas

摘要

摘要: 为研究miR-1和miR-206在不同饲养方式下AA肉仔鸡肌肉组织中的表达情况，本试验采用生物信息学方法比对了miR-1、miR-206在不同物种中的同源性，通过茎环结构实时定量RT-PCR法分析miR-1和miR-206在不同饲养方式下AA肉仔鸡的肌肉组织中的表达，从而初步探究其表达规律。结果表明，gga-miR-1和gga-miR-206成熟序列分别位于鸡基因组20号和3号染色体上；pre-gga-miR-1与eca、ptr、efu、tgu、ola同源性高达86%,pre-gga-miR-206与bta、cfa、hsa、mml、ptr、efu、xtr同源性高达88%;gga-miR-1和gga-miR-206在幼鸡腿肌中表达量高于笼养鸡和散养鸡，且笼养鸡中gga-miR-1和gga-miR-206表达量高于散养鸡，在散养鸡的翅肌和胸肌中表达量高于幼鸡和笼养鸡。表明不同饲养方式下miR-1和miR-206在不同组织表达不同，导致肉品质不同。本研究有助于了解不同饲养方式下miR-1和miR-206表达规律，养殖户可根据不同需求采取相应的饲养方式。
- miR-1 /
- miR-206 /
- 肌肉组织 /
- 骨骼肌 /
- 饲养方式
Abstract: In order to study the expression of miR-1 and miR-206 in the muscle tissue of AA broilers under different feeding methods, this study used bioinformatics methods to compare the homology of miR-1 and miR-206 in different species. The stem-loop real-time quantitative RT-PCR method was used to analyze the expression of miR-1 and miR-206 in the muscle tissue of AA broilers under different feeding methods, so as to initially explore their expression rules. The results showed that the mature sequences of gga-miR-1 and gga-miR-206 were located on chromosomes 20 and 3 of the chicken genome, respectively; pre-gga-miR-1 had high homology（86%） with eca, ptr, efu, tgu, and ola, pre-gga-miR-206 had 88% homology with bta, cfa, hsa, mml, ptr, efu, xtr; gga-miR-1 and gga-miR-206 were highly expressed in young chicken leg muscles. The expression levels of gga-miR-1 and gga-miR-206 in caged chickens were higher than those of free-range chickens, and the expression levels in the wing and breast muscles of free-range chickens were higher than those of young chickens and Cage chickens. It showed that miR-1 and miR-206 were expressed differently in different tissues under different feeding methods, resulting in different meat quality. This study was helpful to understand the expression patterns of miR-1 and miR-206 under different feeding methods, and farmers could adopt corresponding feeding methods according to different needs.
- miR-1 /
- miR-206 /
- Muscle tissue /
- Skeletal muscle /
- Feeding method

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