Abstract: To investigate the effect of dandelion flavonoids on antioxidant enzyme activity and gene expression in mice, fifty 5-week-old SPF ICR male mice（22 g±2 g） were divided into a while the control group, control group, high concentration of dandelion flavonoid group(100 mg ·kg^-1 ·d^-1), medium concentration of dandelion flavonoid group(50 mg ·kg^-1 ·d^-1), low concentration of dandelion flavonoid group(25 mg ·kg^-1 ·d^-1), test group was given different concentrations of dandelion flavonoid once a day by regular gavage, while the control group was given the same amount of normal saline for 4 weeks, control group was given the same amount of normal DMSO for 4 weeks.The activity of superoxide dismutase（SOD） and glutathione peroxidase（GSH-PX）, total antioxidant capacity（T-AOC）, and malondialdehyde（MDA） content were determined in the serum and liver tissues of mice, and the relative mRNA expression of SOD-1, SOD-2, GPX-1, GPX-4 in liver tissues of mice were determined by RT-qPCR. The results show that dandelion flavonoids can significantly increase the activities of SOD, GSH-PX, T-AOC in mouse serum and liver tissue increased significantly（P＜0.05）, the content of MDA reduced significantly（P＜0.05）, and the expression of SOD-1, SOD-2, GPX-1, GPX-4 mRNA increased significantly. Research shows, dandelion flavonoids can improve the antioxidant capacity of mice and have anti-aging effects.