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棉铃虫田间种群Bt抗性监测方法综述

Monitoring Methods of Bt Resistance in Field Population of Helico-verpa armigera

  • 摘要: 迫于苏云金杆菌(Bacillus thuringiensis,Bt)毒素的选择压力,棉铃虫(Helicoverpa armigera)田间种群对Bt毒素产生了抗性。为了研究棉铃虫田间种群Bt抗性,研究者们开发出了一系列棉铃虫抗性监测技术。本文介绍了用于棉铃虫Bt抗性监测常用的生物测定法和分子检测法的原理、应用及优缺点等。虽然目前棉铃虫田间种群Bt抗性监测多采用以F1代筛选法和F2代筛选法为代表的生物测定法,但分子检测法正以其优势在棉铃虫Bt抗性检测中占据越来越重要的位置。本综述期望为读者选择合适的棉铃虫Bt抗性监测方法提供参考和建议。

     

    Abstract: Helicoverpa armigera under the selection pressure of Bacillus thuringiensis toxin has developed resistance to Bt toxin in the field population. In order to study the Bt resistance in the field population of H. armigera, a series of resistance monitoring techniques were developed. This article introduces the principles, applications, advantages, and disadvantages of the bioassay methods and the molecular detection methods commonly used for monitoring Bt resistance of H. armigera. At present, the bioassay methods such as the F1 screen and the F2 screen are mostly used to monitor the Bt resistance in the field population of H. armigera, but the molecular detection methods with their advantages are playing an increasingly important role in the detection of Bt resistance of H. armigera. This review is expected to provide references and suggestions for readers to choose a suitable method of monitoring Bt resistance of H. armigera.

     

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