Abstract: AP2/ERF transcription factors exist in plants and have important regulatory effects on growth, development and response to stress. In order to study the properties of AP2/ERF transcription factor family members in Apocynum venetum, the AP2/ERF transcription factors in A. venetum were identified and analyzed based on the whole genome data and bioinformatics methods. A total of 102 AP2/ERF transcription factors were identified in A. venetum. Combined with conserved domain characteristics and Arabidopsis thaliana family member sequence alignment, they can be divided into 4 subfamilies: AP2, RAV, ERF and DREB. According to the analysis of bioinformatics, the members of this family are hydrophilic proteins, rich in acidic amino acids, and most of the members are non-secreted proteins. According to chromosome location, 100 genes are unevenly distributed on 11 chromosomes of Apocynum venetum, and AP2/ERF genes have tandem duplication on multiple chromosomes. Microarray expression analysis showed that 102 members play an important role in plant growth and development and stress response. The AP2/ERF transcription factors can be induced by low temperature, high temperature, light, shading, ultraviolet and Abscisic acid（ABA） treatment, and can be expressed in roots, stems, leaves, flowers, fruits and seeds. At the same time, according to functional connection network analysis and promoter analysis results of 102 AP2/ERF transcription factors of A. venetum, it is speculated that members of this gene family play an important role in the growth and development of A. venetum and the physiological response to various stresses such as low temperature, drought and salt stress. This study will lay the foundation for the subsequent function verification of the AP2/ERF gene family of A. venetum, and it will also have important significance for the research on the stress resistance function of the AP2/ERF transcription factor family in other species.