Abstract: In this study, microarray data from both eutopic endometrium of adenomyosis patients and endometrium of controls were obtained from the GEO database. Bioinformatics method was used to screen DEGs, and GO enrichment analysis and KEGG enrichment analysis were conducted for these DEGs. Then, PPI network was applied to screens key genes, and the expression levels of selected key genes were verified in tissue samples. In this study, a total of 509 DEGs were screened（P＜0.05, fold change＞2）. GO enrichment analysis showed that DEGs was mainly related to protein localization to endoplasmic reticulum, translation initiations, nuclear transcribed mRNA catabolic process, and multi-organism metabolic process. KEGG enrichment analysis showed that DEGs were mainly concentrated in ribosome signaling pathway, oxidative phosphorylation pathway, Parkinson disease signaling pathway, Alzheimer disease signaling pathway and Huntington disease signaling pathway. The PPI network selected out 10 key genes as RPL35, RPL22, RPL39 L, RPL26 L1, RPS25, RPS29, RPS24, SEC61 A1, SEC61 G and FAU. Results of RT-qPCR and Western blotting showed that the relative expression levels of mRNA and protein of RPL35, RPS25 and FAU were lower in case group, the differences were statistically significant compared with control group（P＜0.05）. This study suggestes that the occurrence of adenomyosis is related to the disorder of ribosomal protein synthesis in the eutopic endometrium, and that low expression of FAU may be involved in the resistance to cell apoptosis of eutopic endometrium cells.