Abstract: To clarify the targeted regulation mechanism and binding sites of PPARγ₂（peroxisome proliferator-activated receptor γ₂） on the MYH7 gene encoding β-myosin heavy chain（β-MHC）. In this study, the pTT5 basic vector and pTT5-3 flag-PPARγ₂ plasmid were transfected into mouse cardiac myocytes（MCM）, respectively, and the expression of MYH7 and PPARγ₂ was detected by RT-qPCR and Western blot. Chromatin immunoprecipitation（ChIP） was used to specifically enrich and purify DNA fragments binding to PPARγ₂ in the MCM, and high-throughput sequencing（HiSeq） was used to detect and analyze genes that can directly bind to PPARγ₂. Relevant bioinformatics software was used to predict PPARγ₂ binding to the promoter of the MYH7 gene, and inserted the predicted transcription factor binding site and corresponding mutation site into the upstream of the luciferase gene in the pgl3-basic vector, respectively. Wild and mutant types of dual-luciferase reporter plasmid were constructed. Meanwhile, they were co-transfected into 293 T cells with pcDNA3.1 vector, pcDNA3.1-PPARγ₂ plasmid and renilla luciferase gene vector pRL-TK. Afterwards, the relative luciferase activity was detected. The results showed that ChIP-seq preliminarily verified that MYH7 was a possible target gene directly regulated by PPARγ₂. The wild and mutant types of dual-luciferase reporter plasmids（pgl3-basic-MYH7-WT and pgl3-basic-MYH7-mut） were successfully built that identified by gene sequencing. Double fluorescence detection results showed that the relative luciferase activity of MYH7-WT+pcDNA3.1-PPARγ₂（2.477±0.212） was higher than that of MYH7-WT+pcDNA3.1（0.366±0.021）, and the difference was statistically significant（P＜0.001）. There was no difference in the relative luciferase activity between groups MYH7-WT+pcDNA3.1-PPARγ₂（2.477±0.212）and MYH7-mut+pcDNA3.1-PPARγ₂（2.677±0.201）（P＞0.05）. In this study, ChIP-seq and dual-luciferase assay technique preliminarily confirm that PPARγ₂ can directly bind to the promoter region of MYH7 and have a regulatory positive effect on the MYH7 gene.