Abstract: Somatostatin（ss） is a type of peptides involved in the regulation of growth, development, reproduction and metabolism in vertebrate. There are six somatostatin gene locis located in the fish genome, and all six genes are existed in zebrafish（Danio rerio）. However, the function of somatostatin was rarely reported in zebrafish. Therefore, we firstly employed in situ hybridization to detect expression pattern of ss1 in the early embynoic development stage of zebrafish, and found that ss1 expressed abundantly in brain, neuron and the pancreas during all the examined developmental stage from 24 hpf to 144 hpf（hours post fertilization）. Its persistant expression pattern in the pancreas during the early developmental stage lead us to hypothesize that ss1 may be involved into the glucose metabolism regulation. Then we exposed 5 dpf（days post fertilization） zebrafish larva to different concentrations of glucose, and found that ss1 mRNA expression level was possitively correlated with the concentration of glucose, and peaked at 5 mg/mL exposure. This results confirmed the hypothesis that ss1 was involved into the regulation of glucose metabolism in the early developmental stage of zebrafish, and provided a preliminary insight into the function and mechanism of ss1 in the glucose homeostasis.