欧美杨细菌性溃疡病菌基因组测序与分析

张立春; 肖丹丹; 付田田; 王延伟

doi:10.13417/j.gab.041.000331

欧美杨细菌性溃疡病菌基因组测序与分析

北京林业大学生物科学与技术学院林木育种国家工程实验室林木花卉遗传育种教育部重点实验室林木分子设计育种高精尖创新中心

基金项目:

国家自然科学基金项目(32071504

31670671)共同资助

详细信息

通讯作者:
王延伟,ywwang@bjfu.edu.cn

中图分类号: S763.7
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出版历程
- 刊出日期: 2022-02-24

Whole-Genome Sequencing and Analysis of Lonsdalea populi Causing Poplar Canker

National Engineering Laboratory for Tree Breeding, Key Laboratory of Ministry of Education for Genetics and Breeding in Forest Trees and Ornamental Plant, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Biotechnology, Beijing Forestry University

摘要

摘要: 欧美杨细菌性溃疡病菌(Lonsdalea populi)是引起杨树溃疡病的主要病原菌，它可引起树干形成层的破坏和树皮的局部坏死。为挖掘其致病基因，本研究利用二代和三代测序技术对欧美杨细菌性溃疡病菌N-5-1进行了全基因组测序。结果表明：该病菌菌株染色体和质粒大小分别为3 832 567 bp和11 289 bp, GC含量分别为55.42%和56.32%。通过对基因组的分析，共预测到3 556个基因，在GO、KEGG和COG数据库分别注释到2 348个、2 785个和2 374个基因。进一步利用Ⅲ型分泌系统数据库注释到501个编码分泌蛋白的基因，在病原互作数据库中注释到了427个病原与宿主互作的基因，其中13个为效应子基因。通过与Lonsdalea属的其他病原菌的基因组进一步比较发现，菌株Lonsdalea populi N-5-1的基因组与Lonsdalea iberica LMG 26264和Lonsdalea populi L2-3的基因组存在良好的共线性关系，且与Lonsdalea populi L2-3的亲缘关系更近。本研究为解析欧美杨细菌性溃疡病菌的基因组结构、功能及亲缘关系提供了科学基础，并为后续深入研究其致病基因及开展树木抗病分子育种提供了基因组信息和基因资源。
- 欧美杨细菌性溃疡病菌 /
- 全基因组测序 /
- 比较基因组学 /
- 杨树 /
- 溃疡病
Abstract: Lonsdalea populi is one of the major pathogenic bacteria causing poplar canker. It can cause the destruction of the cambium in trunks and local necrosis of the bark. In order to explore the pathogenic genes, the complete genome of Lonsdalea populi N-5-1 was sequenced and assembled in this investigation. Results showed that L. populi N-5-1 carries a chromosome of 3 832 567 bp with 55.42% GC and a plasmid of 11 289 bp with 56.32% GC. Through genomic analysis, 3 556 genes were predicted, and 2 348, 2 785, and 2 374 genes were annotated in the GO, KEGG and COG databases, respectively. 501 genes encoding type Ⅲ secreted protein were found using T3 SS database. 427 genes involved in pathogen-host interaction were annotated using PHI database, including 13 genes encoding effectors. After being compared with other pathogens in the Lonsdalea genus, the genome of L. populi N-5-1 strain was found to have a good synteny with those of Lonsdalea iberica LMG 26264 and Lonsdalea populi L2-3, and be more related to that of Lonsdalea populi L2-3. This investigation provides a useful basis for understanding the structure, function and relationship of the genome of Lonsdalea populi, and genomic information and gene resources for the further study of the pathogenic genes and molecular breeding of trees with resistance to pathogens.
- Lonsdalea populi /
- Whole-genome sequencing /
- Comparative genomics /
- Poplar /
- Canker

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