Abstract: Rice（Oryza sativa） bacterial leaf streak is a major rice disease, but the main gene of resistance to bacterial leaf streak has not been located. Therefore, it is of great significance to explore the resistance genes of bacterial leaf streak and provide new gene resources for resistance breeding. In this study, 207 rice varieties were identified by genome-wide association analysis. The general linear model（GLM） and mixed linear model（MLM） were used for association analysis. Seven resistance phenotype associated loci were obtained by using GLM model analysis, which were distributed on chromosomes 2, 3, 8, 11 and 12. Four resistance associated loci were obtained by using MLM model analysis. Among these associated loci, 2 were co-localized with the previously identified loci, and 5 were novel bacterial leaf streak resistance associated loci. Further, we identified 28 candidate genes at the loci that were obtained in both models. These genes not only provide novel gene resources for breeding rice cultivars with bacterial leaf streak resistance, but also laid solid base for further studying the molecular mechanism of bacterial leaf streak resistance in rice.