Abstract: NAC protein is one of the largest family of plant-specific transcription factors and plays an important role in plant response to biological and abiotic stresses. In this study, the second-generation transcriptome sequencing was used to obtain the transcriptome data of Cinnamomum kanehirai under drought and low temperature stress. Local BLAST was used to obtain 78 CkNAC transcription factor sequences from genomic data, and analysis software was used to analyze the conserved motif. The transcriptome analysis was used to obtain CkNAC associated with drought and low temperature stress, and the phylogenetic and related genes were verified by fluorescence quantitative PCR. The analysis of NAC gene expression profiles showed that 18 genes, accounting for 23.1% of the NAC family of C. kanehirai, had significant expression differences under drought stress and low temperature stress. Among them, 10 genes showed high expression level under drought stress, accounting for 55.6% of the up-regulated genes. The phylogenetic tree was constructed by using 14 NAC transcription factors and 8 NAC proteins with drought and cold resistance, according to the results of 22 NACs protein intoⅠ,Ⅱ two categories, gene involved in drought and low temperature stress concentrated inⅠclass. In the Ⅰ class, CkNAC14,CkNAC23,CkNAC44 and CkNAC70 were clustered with TaNAC67 of Triticum aestivum, HanAC38 of Haloxylon ammodendron, ANAC072 of Arabidopsis thaliana and GmNAC20 of Glycine max involved in drought stress, CkNAC44 and CkNAC70 had 59.0% and 58.2% similarity with AnAC072 and GmNAC20. It was speculated that CkNAC14, CkNAC23, CkNAC44 and CkNAC70 might be involved in regulating the response of Cinnamomum kanehirai to drought stress. RT-qPCR was used to detect the expression changes of NAC gene in C. kanehirai under different stress conditions, and the results showed that CKNAC14, CKNAC23, CKNAC44 and CKNAC70 were significantly up-regulated by drought stress. Based on the high-throughput transcriptome data of C. kanehirai, this study explored the biological characteristics of the NAC transcription factor family of C. kanehirai, and provided a scientific basis for further study on the regulation of abiotic stress of the NAC gene of C. kanehirai.