Abstract: The encoding protein of human TAGLN gene is involved in cytoskeleton remodeling and plays an important role in the biological processes of tumor cell proliferation, migration, invasion and metastasis. In order to explore the promoter region, CpG island, transcription factor binding site, post-translational modification site, subcellular localization and B-cell linear epitope of TAGLN gene, the promoter region, CpG island and transcription factor binding sites, post-translational modification sites, subcellular localization and B cell linear epitopes of TAGLN gene were analyzed online by various bioinformatics methods. The results showed that no obvious promoter region was showed by Promoter 2.0;The 2 000 bp region of human TAGLN gene 5′ regulatory region contained promoter sequence with the other three softwares, but the core promoter regions were different of human TAGLN gene corresponding to different transcripts. No CpG island of human TAGLN gene 5′ regulatory region was showed by the four online softwares, but a CpG concentrated distribution region was showed, respectively. Seventeen transcription factor binding sites in common with the promoter region of TAGLN gene were showed in the database of Alibaba 2.1 and PROMO software; Six transcription factors with the same transcription direction（positive chain） were obtained by JASPAR software; The transcription factors JUNB, SMAD4, SMAD1 and SAMD3 were showed in the first promoter bind to the promoter region and were consistent with the transcription direction by Swissregulon; The protein encoded by human TAGLN gene can be modified by phosphorylation, acetylation, ubiquitination and methylation; It is mainly located in cytoplasm and nucleus; there are nine hot spots of B cell linear epitope（antigen epitope）. The promoter region, CpG island, transcription factor binding site, post-translational modification site, subcellular localization and B-cell linear epitope of human TAGLN gene were predicted by bioinformatics methods, the relevant information of the gene can be efficiently obtained, which will provide a theoretical basis for further study of the function, mechanism and antibody development of human TAGLN gene in breast cancer.