Abstract: NAC（NAM, ATAF, and CUC） transcription factor family is found only in land plants and plays important roles in development, stress responses, and biosynthesis. The functions of many NAC family members have been identified（primarily in model plants）, but the evolution of the NAC family in early land plants like bryophytes is not clear. In this study, we analyzed the evolution of the NAC gene family using 97 NAC genes in four bryophyte genomes（the mosses Physcomitrella patens and Sphagnum fallax, the liverwort Marchantia polymorpha, and the hornwort Anthoceros angustus）. Mosses have about 40 NAC genes and liverworts and hornworts have only about 10 NAC genes. In bryophytes, the NAC family is divided into five subfamilies. Analysis of gene structure and conserved domains showed that most NAC genes in the same subfamily had similar gene structures and encode similar protein motifs. Collinear analysis showed that the NAC-Ⅱ gene was located at the conserved collinear region of mosses and expanded with the entire gene repeat event in mosses. These results indicate that the expansion of NAC gene occurs not only in vascular plants but also in bryophytes, which is crucial for functional extension of the NAC gene family. Further studies showed that the amplification of the NAC gene family is closely related to the whole genome duplication（WGD） event in mosses, suggesting that the expansion time of the NAC gene family is accompanied by the whole gene duplication event.