Abstract: TCP family proteins are unique transcription factors in plants and play an important role in plant development. The ZjTCP4 gene was cloned from Zoysia japonica in this study（GenBank accession number: MZ540773）. The complete coding region of the gene was 2 097 bp, encoding 699 amino acids. The analysis of evolutionary tree and conserved sequence showed that ZjTCP4 protein contained TCP domain of highly conserved helix-loop-helix in TCP family, which had the highest homology with ZmTCP4 protein in Zea mays and had similar conserved motif composition. ZjTCP4 belongs to the CIN subclass of the TCP transcrition factor family. According to the results of yeast self-activation, the ZjTCP4 does not have self-activation activity, so it can be used for yeast two-hybrid experiments and protein detection and analysis. The expression pattern analysis of the ZjTCP4 gene showed that it was highly expressed in aged leaves and roots of Zoysia. The subcellular localization of ZjTCP4 protein in Nicotiana tabacum indicated that the protein was localized in the cytoplasm. This study provides a scientific basis for further study of Zoysia japonica ZjTCP4 protein and TCP transcription factor family.