Abstract: To investigate the clinical value of laser artificial shrinkage on the clinical outcome in vitrified-thawed blastocyst cycle, a total of 449 cycles of blastocyst vitrification were retrospectively analyzed in the reproductive medical and genetic center, People’s Hospital of Guangxi Zhuang Autonomous Region from January 2014 to July 2016. According to whether the blastocysts were artificially shrunk by laser before freezing, we divided them into laser artificial shrinkage group（193 cases） and non shrinkage group（256 cases）. The survial rate of blastocysts, biochemical pregnancy rate, clinical pregnancy rate, embryo implantation rate, early abortion rate, multiple pregnancy rate, live birth rate and other clinical outcome indicators of the two groups were compared. The results showed that there were no significant difference in female age, endometrial thickness, survival rate of blastocysts and average number of transferred embryos between the laser artificial shrinkage group and non shrinkage group（P＞0.05）. The biochemical pregnancy rate（63.21%）, clinical pregnancy rate（56.99%） and live birth rate（50.78%） in the laser artificial shrinkage group were higher than those in the non shrinkage group（49.41%, 45.88%, 39.23%）, and the differences were statistically significant（P＜0.05）. The conclusion of this study shows that laser artificial shrinkage has a certain clinical application value in the vitrification cycle of blastocysts and can improve the clinical outcome.