Abstract: To investigate the effects of ubiquitin-like with PHD and ring finger domains 1（UHRF1） on the cellular biology of prostate cancer PC-3 cells and underlying regulatory mechanisms, we transfected PC-3 cells with lentiviruses containing UHRF1 and shRNA-UHRF1 respectively, to construct stable transfected cell lines of UHRF1 silencing or overexpression. The expression level of UHRF1 and the effects of UHRF1 on DNMT1 and p16^{INK4 A} expression at mRNA and protein levels were detected by real-time PCR（RT-qPCR）, Western blot and immunofluorescence experiments, respectively. The effects of UHRF1 on the proliferation, migration, invasion, apoptosis and cell cycle distribution of PC-3 cells were studied by CCK-8, scratch assay, Transwell experiment and flow cytometry, respectively. Our data showed that overexpression of UHRF1 promoted cell proliferation, invasion and migration in PC-3 cells, inhibited cell apoptosis, regulated cells in G2/M phase, increased DNMT1 and decreased p16^{INK4 A} expression at mRNA and protein levels. Downregulation of UHRF1 produces the opposite results. In summary, our results suggested that UHRF1 promote proliferation and migration but inhibits apoptosis of prostate cancer cells, likely by downregulating p16^{INK4 A}. UHRF1 may be a potential therapeutic target for prostate cancer.