Abstract: In this study, the COⅠ, 16S rRNA and 18S rRNA genes from 30 species in 9 genera within Tetriginae were amplified and sequenced. The base composition and interspecies genetic distance of the combined sequence of COⅠ, 16S rRNA and 18S rRNA genes, and the molecular phylogenetic relationships were reconstru-cted using maximum likelihood（ML） and bayesian inference（BI） methods. The main results were as follows:（1） The genetic distances of 30 Tetriginae species ranged from 0 to 0.134. Among them, the genetic distance between Tetrix grossifemura and Tetrix interrupta was 0, and the genetic distance between Bannatettix serrifemoralis and Ergatettix serrifemoroides was 0.001. So Tetrix grossifemura and Tetrix interrupta, Bannatettix serrifemoralis and Ergatettix serrifemoroides were synonymy. In addition, the genetic distance between Ergatettix serrifemoroides and Tetrix grossifemura, Ergatettix serrifemoroides and Tetrix interrupta were 0.005; and the genetic distance between Bannatettix serrifemoralis and Tetrix grossifemur, Bannatettix serrifemoralis and Tetrix interrupta, Teredorus brachinota and Teredorus wuyishanensis were 0.006, they may also be synonymy.（2） The research supported the monophyletic of Teredorus and did not support the monophyletic of Euparatettix, Tetrix, Ergatettix, Bannatettix, Coptotettix, Hedotettix, Formosatettix, Alulatettix at the molecular phylogenetic level. In addition, the main reasons why the current taxonomy system of Tetriginae was not supported at the level of molecular phylogenetic were analyzed from the perspective of morphological taxonomy in this paper. This research will provides a scientific reference for the in-depth classification and revision of Tetrigoidea.