Abstract: To systematically understand the members and characteristics of the YABBY gene family in broomcorn millet（Panicum miliaceum L.）, based on its genome and annotation data, bioinformatics method were adopted to analyze and identify members of gene family, chromosomal distribution, protein characteristics, gene structure, evolutionary relationships, conserved domains, cis-elements of promoter region and expression characteristics under hypertonic solution stress. There are 17 YABBY genes in broomcorn millet, which are distributed on chromosomes 1, 2, 3, 4, 5, 6, 12, 15 and 16. The length of PmYABBY protein ranges from 125 to 935 amino acids and the isoelectric point is between 5.70 and 10.33. The family members contain 3 to 13 introns. By cell localization prediction, all the members were located in the nucleus. MEGA7.0 software was adopted to perform the phylogenetic analysis of YABBY gene families in Arabidopsis thaliana, Oryza sativa, Zea mays, Setaria italica and Panicum miliaceum, showing that the 17 YABBY genes were divided into four subfamilies in broomcorn millet, namely, YAB1/YAB3, YAB2, CRC and INO. Using PlantCare software to analyze the cis-acting elements in the 2 000 bp sequence of YABBY gene in broomcorn millet found that there were a large number of cis-acting elements with different types and numbers, such as light response and hormone response, in the 2 000 bp sequence upstream of the YABBY gene, indicating that the gene expression of this family was closely related to light and hormone factors. Analysis of transcriptome sequencing data under PEG-6000 hypertonic solution stress at different time points in seedling stage showed that PmYABBY5, PmYABBY8, PmYABBY12, PmYABBY7, PmYABBY13, PmYABBY2, PmYABBY9 and PmYABBY7 had obvious change in aboveground organs（stems and leaves） and underground organs（roots）, respectively, indicating that members of YABBY gene family were involved in abiotic stress response. These results provided scientific basis for further functional dissection and genetic utilization of PmYABBY genes in broomcorn millet.