A Comparative Analysis of the Liver Transcriptomes of the Tadpoles of the Microhyla fissipes and Fejervarya multistriata with the Same Domain Distribution
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摘要: 饰纹姬蛙(Microhyla fissipes)蝌蚪和泽陆蛙(Fejervarya multistriata)蝌蚪是南方常见的无尾两栖类，两者同域分布，但在体型和发育分期上差异较大。为深度挖掘两者的肝脏功能基因和物种共存的内在机制，本研究通过Illumina Hiseq平台对发育到38期的饰纹姬蛙蝌蚪和泽陆蛙蝌蚪肝脏进行转录组测序。基因表达差异分析显示，泽陆蛙蝌蚪和饰纹姬蛙蝌蚪肝脏组织中存在3 202个差异基因。相较于饰纹姬蛙蝌蚪，泽陆蛙蝌蚪肝脏中1 635个Unigenes上调、1 567个下调。具体而言，与泽陆蛙蝌蚪相比，在饰纹姬蛙蝌蚪肝脏中编码抗氧化酶和热休克蛋白以及与热应激相关的基因表达均升高，推测两种蝌蚪可能通过热生态位分离，从而有效减轻或避免有限资源下的种间竞争。本研究为探讨同域分布的不同物种之间的共存机制提供重要的参考依据。Abstract: The tadpoles of Microhyla fissipes and Fejervarya multistriata are common anura in southern China. They are distributed in the same domain, but their size and developmental stages are quite different. In order to explore the functional differences and mechanisms of coexistence between the two species, the Illumina Hiseq was used to sequence the transcriptome of the liver of Microhyla fissipes and Fejervarya multistriata tadpole at stage 38. There were 3 202 differentially expressed genes in the liver of the two tadpoles, of which 1 635 Unigenes were up-regulated and 1 567 Unigenes were down-regulated in Fejervarya multistriata compared to Microhyla fissipes. The expression level of the genes encoding antioxidant enzymes, heat shock proteins and heat stress-related genes in the liver of the Microhyla fissipes was higher than that of the Fejervarya multistriata, suggested that there were different in the temperature tolerance and thermal tolerance ability between the two tadpoles, and they might effectively reduce or avoid the interspecies competition under limited resources through thermal site separation. This research provides an important reference basis for exploring the coexistence mechanism of different species distributed in the same region.
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Keywords:
- Microhyla fissipes /
- Fejervarya multistriata /
- Tadpole /
- Liver /
- Transcriptome
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