Abstract: WOX（WUSCHEL-related homebox）, an unique type of transcription factors in plant, plays signi-ficant roles in regulating plant growth and development and the response to abiotic stress. Neolamarckia cadamba is an important fast-growing tree species in South China with high economic value. In order to characterize the functions of WOX in N.cadamba, genome-wide analysis of WOX gene family was conducted in this study using bioinformatic method. The results showed that a total of 19 NcWOX genes was identified and their protein-related information, gene structure, chromosome distribution, phylogenetic relationship and gene expression patterns were subsequently analyzed. The NcWOX genes were distributed across 14 chromosomes, and their proteins were divided into three groups by phylogenetic tree, including WUS clade, intermediate clade and ancient clade. Multiple sequence analysis of the NcWOX gene family reveals a highly conserved homeodomain. Expression profile analysis shows that NcWOX4.1、 NcWOX4.2、 NcWOX13.1 and NcWOX13.2 have high expression levels in vascular. Particularly, NcWOX4.1、 NcWOX4.2 exhibited the highest expression levels in TCA（transition phase of cambium）. These results indicated that NcWOX4.1、 NcWOX4.2、 NcWOX13.1 and NcWOX13.2 might be involved in vascular development in N. cadamba. Analysis of the promoter elements of the NcWOXs showed that there were rich ele-ments in response to light, hormone and stress. Collectively, identification and preliminarily analysis of WOX gene family members in N. cadamba has been conducted in present study, which lay a theoretical basis for the further functional investigation of NcWOX genes in N. cadamba.