Abstract: The study aims to investigate the effects of miR-192-5 p on proliferation, migration and invasion of pancreatic cancer cell, and to provide an experimental basis for clinical diagnosis and therapy of pancreatic cancer. Real-time quantitative PCR（RT-qPCR） was used to detect the expression level of miR-192-5 p in human normal pancreatic epithelial cell hTERT-HPNE and pancreatic cancer cell AsPC-1. The effects of miR-192-5 p on the proliferation, clone formation, migration and invasion of AsPC-1 cells were analyzed by CCK-8（Cell Counting Kit-8）, colony formation, wound healing and Transwell assays, respectively. Bioinformatics methods were used to predict the candidate target gene of miR-192-5 p. Double luciferase reporter vector assay was employed to verify the targeting relationship between miR-192-5 p and its target gene. RT-qPCR was used to detect the effect of miR-192-5 p on the expression of ZEB2 mRNA. The results showed that the expression of miR-192-5 p in pancreatic cancer cell AsPC-1 was significantly lower than that in human normal pancreatic epithelial cell hTERT-HPNE. The expression of miR-192-5 p was increased significantly in AsPC-1 cells trans fected with miR-192-5 p mimics, and miR-192-5 p mimics significantly inhibited the expression of ZEB2 mRNA, the proliferation, clone formation, migration and invasion of AsPC-1, compared with mimics NC. The expression of miR-192-5 p was decreased significantly in AsPC-1 cells transfected with miR-192-5 p inhibitor, and miR-192-5 p inhibitor significantly promoted the expression of ZEB2 mRNA, the proliferation, clone formation, migration and invasion of AsPC-1, compared with inhibitor NC. Double luciferase reporter vector assay showed that ZEB2 was the functional target gene of miR-192-5 p. Our findings suggested that miR-192-5 p might inhibit proliferation, clone formation, migration and invasion of pancreatic cancer cell line AsPC-1 by targeting ZEB2.