Abstract: Plukenetia volubilis L. is one of the promising woody oil crops, but its complete and effective gene-tic transformation system has not been established, which seriously restricts the application process of molecular breeding technology of P. volubilis. BABY BOOM（BBM） is a transcription factor widespread among almost all plants, and is believed to play an important regulatory role in plant embryonic development and plant regene-ration. In this study, PvoBBM, a homologs of AtBBM in Arabidopsis thaliana, was cloned from Plukenetia volubilis, and its expression characteristics and functions were studied, which laid the foundation of using this gene to promote transgenic breeding of P. volubilis. The members of AINTEGUMENTA-LIKE（AIL） family were screened from the genome sequence of P. volubilis by local BLAST, PvoBBM was identified by multiple sequence alignments and phylogenetic analysis, and this gene was cloned and constructed into the expression vector. The expression pattern of PvoBBM was analyzed by using RT-qPCR, and this gene was transferred into Arabidopsis thaliana and Nicotiana benthamiana hairy roots for functional studies. There were 11 members of AIL genes family in Plukenetia volubilis, phylogenetic analysis showed that the member xingyouteng＿10009416.1 had the closest relationship with AtBBM, the types and numbers of conserved motifs of their encoded proteins were basically the same, and the similarity of their amino acid sequences was 43.7%, which was named PvoBBM. It was showed that PvoBBM was highly expressed in the young fruits and top of cymes of P. volubilis. Overexpression of PvoBBM could promote the dedifferentiation of growing point and hypocotyl-root transition zone of transgenic Arabidopsis thaliana, and a small number of transgenic plants spontaneously formed regeneration buds in hormone-free medium, but the proportion in T0 and T1 generations was low. A large number of transgenic hairy roots were obtained by Agrobacterium rhizogenes K599 mediated genetic transformation using Nicotiana benthamiana leaves as transformation materials. No dedifferentiation was found in the obtained transgenic hairy roots, but transgenic regenerated buds were induced on the leaves cultured in vitro. In this study, PvoBBM, a morphogenetic gene with the function of promoting shoot regeneration, was cloned from Plukenetia volubilis, which provided a high-quality candidate gene for establishing the genetic transformation system of P. volubilis.