Abstract: To investigate the characteristics of NnPRR family of pseudo-response regulators（PRR） in lotus（Nelumbo nucifera） and their role in abiotic stress response, seven NnPRR genes were identified from the whole genome of N. nucifera by bioinformatics analysis, and their gene structure, protein conserved motif, cis-acting element and collinear relationship were identified and analyzed. The expression patterns of each gene under treatments of the exogenous 300 mmol/L NaCl, 5 mmol/L salicylic acid（SA） and low temperature at 4 ℃ were explored by real time quantitative PCR. The results showed that this family genes could be divided into three branches, which were concentrated on chromosomes chr2,chr3,chr5 and chr6, and the proteins of this family were all located in the nu-cleus. Great numbers of light response elements has been found in their promoter regions, and some of the genes also contain the specific response elements such as low temperature, methyl jasmonate and abscisic acid, ect. From the gene expression pattern, seven genes in NnPRR gene family were positively responsive to exogenous NaCl treatment, while NnPRR2, NnPRR4 and NnPRR3 genes specifically responded to exogenous SA or low temperature stress, suggesting that this family genes were specifically involved in abiotic stress in N. nucifera. The results of this study not only provide a theoretical basis for in-depth study of the response to stress of N. nucifera PRR gene family, but also provide new gene resources for the breeding of new stress-resistant varieties.