Abstract: Strain LN-1 is isolated from long-term tobacco planting soil, which can degrade nicotine efficiently. In order to further clarify the nicotine degradation mechanism of strain LN-1, strain LN-1 was subjected to whole genome sequencing in this study. The whole genome sequence of strain LN-1 was obtained. The taxonomic position of strain LN-1 was determined by phylogenetic analysis based on 16S rRNA gene sequence and average nucleotide identity analysis. The strain LN-1 was identifiled as Agrobacterium radiobacter. Through genome analysis, it was found that the gene cluster involved in nicotine degradation was located on the chromosome 2 of strain LN-1. Meanwhile, chromosome 2 of LN-1 also harbored a p-hydroxybenzoic acid degradation gene cluster, which contained genes similar to the known p-hydroxybenzoic acid degradation genes（pcaG, pcaH, pcaC, pcaQ, pobA, pcaR, pcaI, pcaJ, pcaF, pcaB, pcaD, pobR）. Therefore, this study also verified the ability of strain LN-1 to degrade p-hydroxybenzoic acid. Strain LN-1 has the ability to degrade both nicotine and p-hydroxybenzoic acid, which has the potential value in improving microecological structure of tobacco planting soil and alleviating continuous cropping obstacles of long-term tobacco planting.