Abstract: The differentially expressed genes（DEGs） in the insulin enhancer binding protein 1（ISL1） gene deletion group and the wild-type group（control group） during cardiovascular development were analyzed in mice（Mus musculus） by bioinformatics to explore the downstream target genes associated with ISL1 expression deficiency and toelucidate the mechanism of ISL1 transcriptional regulation of cardiovascular development. The sequencing results of GSE80383 and GSE65658 were downloaded from the GEO database, and the DEGs between the ISL1 expression deletion group and the control group were screened by bioinformatics analysis（P＜0.05, |log₂FC|≥2）. DAVID and STRING online analysis software were used for function enrichment analysis and protein-protein interaction（PPI） analysis of DEGs, respectively. The results showed that ISL1 expression deficiency in mice embryonic day 8.75（E8.75） mainly regulated proximal/distal pattern formation mediating cardiovascular development. ISL1 expression deficiency in mice embryonic day 10.5（E10.5） mainly regulated biological processes such as cell proliferation, differentiation and multicellular organisms, and ISL1 mediated multiple sinoatrial node-associated genes regulating sinoatrial node cell development. The research summarized the role of murine ISL1 in cardiovascular development and outlined its possible transcriptional regulatory network, thus providing a theoretical basis for the molecular basis of cardiovascular disease and cardiac regeneration.