Abstract: The puropse of this study was to investigate the effects of total anthraquinone of Zijiuniu on proliferation, apoptosis, migration and invasion of human（Homo sapiens） gastric cancer SGC7901 cells and its correlation with PI3K/AKT/mTOR pathway. After treating the cells with different concentrations of total anthraquinone of Zijiuniu for 48 h and 72 h, proliferation capacity of SGC7901 cells were detected by CCK-8 method. Apoptosis was detected by Hoechst cell staining and Annexin V-FITC/PI staining. Migration and invasion were detected by scratch test and Transwell test. Apoptosis related proteins Cleaved-Caspase-3, BAX, BCL-2 in the mitochondrial pathway, migration and invasion related proteins MMP-2, MMP-9 and PI3K/AKT/mTOR signaling pathway associated proteins expression were detected by Western blot. The results showed that compared with the control group, the total anthraquinone of Zijiuniu had a significant inhibitory effect on the proliferation of SGC7901 cells（P＜0.01）, the inhibition rate was positively correlated with drug concentration and action time, the half inhibitory concentration(IC₅₀) of 48 h and 72 h were 190 and 120 μg/mL respectively. The total anthraquinone of Zijiuniu on centration at 100 and 50 μg/mL can induce apoptosis in SGC7901 cells. The total anthraquinone of Zijiuniu on centration at 100, 50 and 25 μg/mL can inhibit the migratory ability of SGC7901 cells（P＜0.01 or P＜0.05）.The total anthraquinone of Zijiuniu on centration at 100 and 50 μg/mL can inhibit the invasion ability of SGC7901 cells（P＜0.01 or P＜0.05）. The total anthraquinone of Zijiuniu at 100 μg/mL can down regulate MMP-2 and MMP-9 proteins expression level（P＜0.05）. The total anthraquinone of Zijiuniu at 100 and 50 μg/mL can up regulate the expression level of Cleaved-Caspase-3 and BAX protein, and down regulate the expression level of p-AKT protein（P＜0.01 or P＜0.05）. The total anthraquinone of Zijiuniu at 100, 50 and 25 μg/mL can down regulate the expression level of p-mTOR and BCL-2 protein（P＜0.01 or P＜0.05）. The present study found that the total anthraquinone of Zijiuniu could inhibit the proliferation of SGC7901 cells, induce the apoptosis, inhibit the migration and invasion, and its effects were related to the inhibition of PI3K/AKT/mTOR signaling pathway.