非靶向脂质组学分析伏马菌素B1对未成熟树突状细胞脂质代谢的影响

唐开义; 于欢; 喻艳琴; 欧沙; 王赟; 曾柱; 胡祖权

doi:10.13417/j.gab.042.000327

非靶向脂质组学分析伏马菌素B1对未成熟树突状细胞脂质代谢的影响

唐开义¹,
于欢²,
喻艳琴¹,
欧沙²,
王赟^1,2,
曾柱^1,2, ,,
胡祖权^2, ,

1. 贵州医科大学基础医学院,贵州省高等学校感染免疫与抗体工程重点实验室;
2. 贵州医科大学生物与工程学院(健康医药现代产业学院),贵州省高等学校免疫细胞与抗体工程研究中心/生物与医学工程重点实验室

基金项目:

国家自然科学基金项目(21906036)

贵州省省级科技计划项目(黔科合基础-ZK[2021]重点029、黔科合平台人才[2021]5637号、黔科合基础-ZK[2022]一般397、黔科合平台人才[2016]5676)

贵州医科大学优秀青年人才培养项目(贵医大优秀青年人才[2021]101号)共同资助

详细信息

通讯作者:
曾柱,zengzhu@gmc.edu.cn

胡祖权,huzuquan@gmc.edu.cn

中图分类号: R994
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出版历程
- 刊出日期: 2023-03-24

Non-targeted Lipomics Analysis of the Effects of Fumonisin B1 on the Lipid Metabolism in Immature Dendritic Cells

TANG Kai-yi¹,
YU Huan²,
YU Yan-qin¹,
OU Sha²,
WANG Yun^1,2,
CENG Zhu^1,2, ,,
HU Zu-quan^2, ,

1. Key Laboratory of Infectious Immune and Antibody Engineering in University of Guizhou Province, School of Basic Medical Sciences,Guizhou Medical University;
2. Immune Cells and Antibody Engineering Research Center in University of Guizhou Province/Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering (School of Modern Industry for Health and Medicine) , Guizhou Medical University

摘要

摘要: 伏马菌素B1 (fumonisin B1, FB1)是一种具有免疫抑制作用的真菌毒素。本研究利用FB1处理未成熟树突状细胞(immature dendritic cells, imDCs),通过非靶向脂质组学分析FB1对imDCs脂质代谢的影响。运用超高效液相色谱串联四极杆静电场轨道阱质谱(UPLC-Q-Exactive Orbitrap-MS)技术采集质谱数据，通过主成分分析(principal component analysis, PCA)、偏最小二乘法判别分析(partial least-squares discrimination analysis, PLS-DA)和正交偏最小二乘法判别分析(orthogonal partial least-squares discrimination analysis, OPLS-DA)进行多维统计分析，比较FB1处理组和对照组之间脂质代谢谱的差异，并以OPLS-DA模型的变量权重值(variable importance for the projection, VIP)>1和P<0.05为标准筛选差异脂质分子。脂质组学分析结果显示，从imDCs中共检测到36个脂质亚类，1 845个脂质分子。从FB1处理的imDCs中筛选出30个显著性差异脂质分子，其中神经酰胺(ceramide, Cer)、磷酸神经酰胺(ceramide phosphate, CerP)、单半乳糖甘油二酯(monogalactosyldiacylglycerol, MGDG)、磷脂酰胆碱(phosphatidylcholine, PC)、鞘氨醇(sphingosine, So)、磷脂酰丝氨酸(phosphatidylserine, PS)和鞘磷脂(sphingomyelin, SM)等亚类的含量显著下降(VIP>1,P<0.05),而甘油二酯(diacylglycerol, DG)和磷脂酰肌醇(phosphatidylinositol, PI)的含量显著升高(VIP>1,P<0.05)。研究结果说明FB1可能通过干扰imDCs的正常脂质代谢来发挥其免疫抑制作用。
- 伏马菌素B1 /
- 树突状细胞 /
- 脂质组学 /
- 脂质代谢物 /
- 鞘脂代谢
Abstract: Fumonisin B1（FB1） is a mycotoxin with immunosuppressive effect. In this study, FB1 was used to treat immature dendritic cells（imDCs）, and non-targeted lipidomics analysis was deployed to analyze the effects of FB1 on the lipid metabolism in imDCs. Mass spectrum data were collected by UPLC-Q-Exactive Orbitrap-MS technology and multivariate statistical analyses were carried out by the principal component analysis（PCA）, partial least squares discrimination analysis（PLS-DA） and orthogonal partial least-squares discriminant analysis（OPLS-DA）. The differences of lipid metabolism profiles between FB1-treated group and control group were compared, and the differential lipid molecules were screened with the criterion of variable importance for the projection（VIP）＞1 in the OPLS-DA model and P＜0.05. The results showed that a total of 36 lipid subclasses and 1 845 lipid molecules in imDCs were detected by the lipidomics analysis. After treatment with FB1, 30 lipid molecules in imDCs with significant differences were screened. Among them, the contents of some lipid subclasses, such as ceramide（Cer）, ceramide phosphate（CerP）, monogalactosyldiacylglycerol（MGDG）, phosphatidylcholine（PC）, sphingosine（So）, phosphatidylserine（PS） and sphingomyelin（SM） decreased significantly（VIP＞1, P＜0.05）, while the levels of diacylglycerol（DG） and phosphatidylinositol（PI） increased significantly（VIP＞1, P＜0.05）. The research results suggested that FB1 may exert immunosuppressive effect by interfering with the normal lipid metabolism in imDCs.
- Fumonisin B1 /
- Dendritic cells /
- Lipomics /
- Lipid metabolite /
- Sphingolipid metabolism

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