Abstract: Solute carrier family 7 member 11（SLC7A11）,plays an essential role in the deposition of melanin in animal skin and hair.Using Illumina HiSeq sequencing,we identified the downstream genes and signaling pathways respond to Rex rabbit（Oryctolagus cuniculus） SLC7A11 in melanocytes.A total of 110 differentially expressed genes（|log2fold change|＞1,P ＜0.05） were identified in melanocytes of overexpression SLC7A11 compared to controls,in which 60 were up-regulated and 50 were down-regulated.GO and KEGG analyses revealed that differentially expressed genes were mainly enriched in iron ion transmembrane transport and lipid metabolism pathways.The expression levels of six differentially expressed genes were validated by real time quantitative PCR,and the trends were consistent with the sequencing results,demonstrating the reliability of the sequencing results.The iron transport-related genes SLC40A1,SCARA5,and lipid metabolism-related genes PLA2G3 and PLA2G2 were further selected.Real time quantitative PCR results showed that overexpression of the SLC7A11 gene significantly down-reguted the expression of SLC40A1,SCARA5,PLA2G3,and PLA2G2（P＜0.05）,while C11 BODIPY 581/591 results indicated that overexpression of SLC7A11 gene inhibited lipid peroxidation in melanocytes.The findings might help researchers better understand how SLC7A11 affects melanocytes.