Abstract: Colloidal gold immunochromatography is a new rapid detection technique using colloidal gold as a tracer marker applied to antigen-antibody reactions,and there are few comparative studies on different interception modes in colloidal gold immunochromatographic techniques for antibody detection at home and abroad.In this study,the gD gene of infectious bovine rhinotracheitis virus（IBRV） was expressed in prokaryote,and the purified IBRV gD-pAb was used as the target of the detection.The colloidal gold-labeled gD protein,staphylococcal protein A（SPA）,streptococcal protein G（SPG）,goat anti rabbit IgG were coated on the gold pad,while the unlabeled above four proteins were respectively coated at the detection line of NC membranes.Colloidal gold immunochromatographic test strips with seven antibody interception modes were prepared by combining gold pads and NC membranes,to evaluate the practical performance of different interception modes by comparing their sensitivity,color development effect and intra-batch reproducibility.The results showed that the sensitivity of labeled antigen-SPG,labeled antigen-SPA and labeled secondary antibody interception mode（indirect method） was about 200 times higher than that of labeled antigen-antigen interception mode（double antigen sandwich method）.The sensitivity of labeled antigen-SPG,labeled antigen-SPA and labeled SPG-antigen,labeled secondary antibody-antigen interception mode in the indirect method was similar,while the sensitivity of labeled antigen-secondary antibody interception mode and labeled SPA-antigen interception mode was reduced with poor color rendering and intra-batch reproducibility due to the properties of IgG-binding protein.Seven antibody interception mode test strips were negative in detecting standard positive serum for Mycobacterium avium subsp.paratuberculosis（MAP）,akabane virus（AKAV）,bovine viral diarrhea virus（BVDV）,Mycoplasma bovis,peste des petits ruminants virus（PPRV）,Brucella,and foot-and-mouth disease virus（FMDV）.The actual performance of IgG-binding proteins was ranked as SPG＞SPA＞secondary antibody.This study provides reference for the preparation of colloidal gold immunochromatographic test strips for antibody detection of other diseases.