Abstract: Solanum lycopersicum PP2C67 gene（SlPP2C67） is an important member of protein phosphatase 2C gene family. In this study, the 1 657 bp promoter sequence of SlPP2C67 was cloned from ′Micro-Tom′. Analysis of cis acting elements of the promoter indicates that SlPP2C67 promoter contained basic regulatory elements, such as CAAT-box and TATA-box, and it also contained multiple tissue specific expression elements, light responsive elements and hormone responsive elements. CaMv35S promoter in pCAMBIA1300-GN vector was replaced by the cloned promoter to construct recombinant expression vector that was transformed into Arabidopsis thaliana for functional verification. GUS histochemical staining showed that the promoter of SlPP2C67 could drive GUS gene to express in A. thaliana leaves specifically. The results of real-time quantitative PCR showed that SlPP2C67 was mainly expressed in tomato leaves, which was consistent with the results of GUS gene expression driven by SlPP2C67 in A. thaliana. This study provides a theoretical basis for further functional research of SlPP2C67 and genetic improvement of tomato.