Abstract: In order to obtain the genome sequence information of Amycolatopsis sp.QT-25, this study predicted its metabolites by analyzing the biosynthetic gene clusters of secondary metabolites, which would provide a scientific basis for the discovery of potential new structural antibiotics. The strain Amycolatopsis sp. QT-25 was identified at the genus level based on the 16S rRNA gene sequence and the genome of Amycolatopsis sp. QT-25 was sequenced by Illumina HiSeq + PacBio sequencing technology. Furthermore, its secondary metabolite biosynthetic gene clusters were analyzed by antiSMASH（v6.0.1） online tool. The results showed that the genome sequence of Amycolatopsis sp. QT-25 was 8 010 145 bp in length, with an average G+C content of 68.66%, containing 7 488 genes. It was predicted that the strain had at least 30 secondary metabolite biosynthetic gene clusters, among which cluster 4 had the potential to encode glycopeptide antibiotics. The results in this study demonstrated that Amycolatopsis sp. QT-25 from Helan Mountain rock lichens has a rich ability of secondary metabolite production, which has the value of further exploring new structural antibiotics.