Abstract: In plants, 1-deoxy-D-xylulose 5-phosphate synthase（DXS） is the first key enzymes in the methy-lerythritol phosphate（MEP） pathway, and plays an important role in the pathways of terpenoid biosynthesis in lavender（Lavandula angustifolia）. In this study, 22 DXS family members in four clades, named LaDXS, were identified in the lavender genome via genome sequence analysis. Most LaDXS were stable proteins dominated by α-helix and random coil, and contained 143～848 amino acids with a molecular weight of 15.6～91.4 kDa. They had no transmembrane domain and signal peptide. Most LaDXS proteins（72%） were localized in chloroplasts. Gene expression analysis showed level difference of LaDXS in tissues of lavender. La23G00913 and La07G01277 were significantly expressed in all tissues except petals, while La11G00786, La17G01254 and La22G01334 had the highest expression in flower bud and calyx, which might be closely related to synthesis of terpenes in lavender essential oil. Promoter analysis revealed that LaDXS gene may be regulated by many factors including light intensity, methyl jasmonate, and abscisic acid, etc. RT-qPCR analysis confirmed that expression of La11G00786, La02G02002, La03G00622, and La23G00913 in flower buds was significantly induced by light. These results will be helpful to further study on the role of LaDXS family genes in the synthesis of lavender terpenoids.