Abstract: Reducing enzyme cost used in lignocellulosic biorefineries is important to promote the green utilization of lignocellulose. β-glucosidase, a rate-limiting enzyme during enzymatic hydrolysis of cellulose, is inhibited by a high concentration of glucose. Mining and mutating the genes of glucose-tolerant β-glucosidases are important strategies. This paper summarizes the progress made in recent years in mining glucose-tolerant β-glucosidase genes, analyzing the molecular mechanism of enzyme tolerance to glucose, mutation of β-glucosidase gene to improve the glucose-tolerance, and genetic engineering of modified microbial strains based on β-glucosidase genes, and then perspectives. This paper will provide references for constructing genetically engineered strains with a high yield of glucose-tolerant β-glucosidase, which is of great significance to the development of lignocellulosic biorefineries.