Abstract: Myelocytomatosis protein 2（MYC2）, as a member of MYC-type bHLH transcription factor family, is a key transcription factor in the jasmonate response pathway and plays an important role in regulating plant resistance to stress. Based on the sequence from wild strawberry（Fragaria vesca） in NCBI database, a FaMYC2-like gene was cloned and identified from strawberry（Fragaria × ananassa） ‘Benihoppe’. The length of its open reading frame was 1 473 bp, encoding 490 amino acid residues. Based on the conserved domains analysis, FaMYC2-like protein had a conserved domain of bHLH-MYC family. Phylogenetic analysis showed that FaMYC2-like protein was closely related to the homologous genes in mostly Rosaceae plants such as Chinese rose（Rosa chinensis）. Through the prediction of cis-acting elements of the promoter, it was found that the promoter region contained a large number of response elements of light signal, stress response and hormone signal. The results of subcellular localization showed that FaMYC2-like protein was mainly located in the nucleus. Result of tissue-specific RT-qPCR showed that the expression of FaMYC2-like gene was highest in the root of strawberry, next was in stem, leaf and flower, with the lowest in the stolon. In different stages of fruit development, FaMYC2-like showed the highest expression in the small green stage and large green stage of early fruit development, and almost no expression in the middle and late fruit development. Low temperature, drought and salt stress all significantly induce the expression of FaMYC2-like in strawberry roots and leaves at early stage.