Abstract: Lipidomics is a new metabolomics technology. Lipids, as important compounds in living organisms, play a crucial role in regulating plant responses to various abiotic and biotic stresses. Lipid metabolism is involved in various reproductive and developmental processes, such as ovule and seed development, callus induction, flowering, and is present in different tissues of various plants, whether in seedlings, pollen, seed coats, or ovule. Over the years, lipid metabolism has been a significant focus in unravelling the molecular mechanisms underlying plant growth and development. This review provides an overview of lipidomics in the field of plant research, with a particular emphasis on summarizing and elucidating how plants remodel lipids in response to various stresses and the potential impact on their morphological and physiological functions.