Abstract: To further mine the transcriptome data of the skeletal muscle of Ira rabbits, bioinformatics was used to conduct an in-depth comprehensive analysis of the transcription data of the gastrocnemius muscle of Ira rabbits at three growth stages in the study. The expression patterns and molecular function of alternative splicing, new transcripts, and their transcription factors were visualized. The results showed that skipping exon（SE） was the most common splicing mode in skeletal muscle growth of Ira rabbits, and the genes for alternative splicing were mainly enriched in ubiquitination-mediated proteolysis. Meanwhile, four new transcripts codes were Novel.10047, Novel.13070, Novel.16785, and Novel.7389 respectively, which were mainly related with the oxidation-reduction process and mitochondrial function in skeletal muscle. Transcription factors including PLAGL1, KLF15, IKZF2, and ZNF566 belonged to the zf-C2H2 transcription factor family may be involved in the regulation of skeletal muscle growth in Ira rabbits as potential targets. The results supplemente the genome and transcriptome annotation information of Ira rabbits and provide a theoretical foundation and basis for further improving and analyzing the potential functional genes and their roles in the skeletal muscle growth of Ira rabbits.