Abstract: Amazona auropalliata has been listed as a protected animal in Appendix Ⅱ of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. In order to better explore the origin, evolution, and phylogenetic status of Amazona auro-palliata in the order Psittaciformes, the direct sequencing method was used to obtain the complete mitochondrial genome sequence of Amazona auropalliata（18 679 bp）, and the structure analysis of the whole sequence was carried out in this study. The results showed that the base content of Amazona auropalliata mitochondrial genome had obvious AT preference, there were 13 protein-coding genes, 22 tRNA-coding genes, and 2 rRNA-coding genes. Except for nad6 on the L chain, other protein-coding genes were on the H chain. The mitochondrial genome of Amazona auropalliata had gene overlap and was closely arranged. The length of the overlapping sequence ranged from 1 bp to 6 bp, and the total length was 17 bp, totaling 8 locations. The length of the intergenic sequence ranged from 1 bp to 40 bp, and the total length is 154 bp, there were a total of 24 locations, with 7 genes arranged without gaps. Based on the mitochondrial gene sequence, analysis found that except for trnC, trnF, trnG, and trnH in tRNA, which lack anticodon loops, trnP and trnQ lack TΨC loops, trnL-Ⅰ, trnS-Ⅱ, and TrnW were short rod-shaped, and other tRNAs had classic clover secondary structure. The phylogenetic tree was constructed using the maximum likelihood method（ML） and neighbor-joining method（NJ） based on the mitochondrial genome sequence of 40 species of Psittaciformes. The results showed that Amazona auropalliata had the closest genetic relationship with Amazona ochrocephala.