Zebrafish hdac10 Improves Cell Survival Under Low Temperature Stress by Promoting Autophagy

HDAC10（histone deacetylase 10） is an important epigenetic enzyme that plays a role in multiple biological processes, such as homologous recombination and regulation of transcription. However, the role of HDAC10 in fish under low temperature stress still remains unclear. In this study, it was showed that the expression of hdac10 in both HEK-293 T cells and ZF4 cells significantly increased under low temperature stress. A zebrafish hdac10 overexpression plasmid was constructed and transfected into HEK-293 T cells. Western blot analysis showed that exogenous hdac10 was successfully expressed in HEK-293 T cells, and the mRNA and protein levels of autophagy related genes, BECN1 and LC3, were significantly upregulated in hdac10 overexpressing HEK-293 T cells, and the survival rate of HEK-293 T cells overexpressing exogenous hdac10 significantly increased under low temperature stress（P＜0.05）, indicating that zebrafish hdac10 overexpression could protect HEK-293 T cells under low temperature stress. Then ZF4 cells were infected with lentivirus expressing hdac10-shRNA, and RT-qPCR showed that endogenous hdac10 was significantly knocked down in ZF4 cells, the mRNA and protein levels of becn1 and lc3 in ZF4 cells were significantly downregulated by hdac10 knockdown, and the survival rate of ZF4 cells under low temperature stress significantly decreased after hdac10 knockdown（P＜0.05）, indicating that hdac10 could protect ZF4 cells under low temperature stress. In conclusion, zebrafish hdac10 upregulates the expression of autophagy related genes and improves cell survival under low temperature stress. This study provides a scientific basis for studying the cold resistance mechanism of fish under low temperature.