Construction of ceRNA Network and Bioinformatics Analyses of Blood Exosomal From Colorectal Cancer

To construct a competitive endogenous RNA（ceRNA） network of circRNA/lncRNA-miRNA-mRNA in blood exosomes of patients with colorectal cancer（CRC）, and predict the potential regulatory mechanisms between differentially expressed genes（DEGenes）, the data of messenger RNA（mRNA）, long non-coding RNA（lncRNA）, circular RNA（circRNA） from the blood of healthy volunteers and CRC patients were downloaded from the exoRBase database. After screening and analyses, a total of 120 mRNAs, 52 lncRNAs and 81 circRNAs were found to be differentially expressed in CRC patients. Multiple databases were used to predict miRNAs that can bind to lncRNA, circRNA and mRNA, and then the ceRNA network of lncRNA/circRNA-miRNA-mRNA was successfully constructed. Gene ontology（GO） analysis showed that DEGenes in the ceRNA network are mainly involved in the biological regulation and structure maintenance of the Golgi apparatus, cell adhesion and connection, and enzyme catalytic activity. The Kyoto Encyclopedia of Gene and Genomes（KEGG） analysis enriched 14 signaling pathways, mainly involved in shigellosis and MAPK signaling pathways. Therefore, this study successfully constructed the ceRNA network of blood exosomes in CRC patients, laying a foundation for exploring the pathogenesis of CRC patients.