Abstract: Pinic acid was prepared via oxidation and bromoform reactions using α-pinene as starting material, and it was converted into dichloride. Then, pinic acid-based diisothiocyanates were synthesized by the reaction of pinic acid dichloride with potassim thiocyanate, followed by N-acylation reaction of pinic acid-based diisothiocyanates with phenyl amines to afford twelve novel pinic acid-based dithioureas. The structures of the target compounds were characterized by means of IR, 1H NMR, 13C NMR, MS, and elemental analysis. The preliminary bioassay showed that the title compounds exhibited a certain herbicidal activity, fungicidal activity, and plant growth regulating activity. Compound E1 displayed growth inhibition activity of 66.6% against root of rape (Brassica campestris) at the concentration of 100 mg/L (B-class activity level).