Abstract: Anti-lipid peroxidation of chlorophyll-carotene paste from pine needles was investigated in vitro. MDA production in rat liver homogenate and mitochondria spontaneously formed or induced by VC-Fe2+ system were measured. The results showed that the chlorophyll-carotene paste could inhibit the generation of MDA in liver homogenate under spontaneous and induced conditions, as well as in liver mitochondria under induced condition, with IC50 of 398.72 mg/L, 355.05 mg/L and 687.02 mg/L, respectively. The swelling of liver mitochondria was also inhibited with dose-response relationship. It is suggested that chlorophyll-carotene paste from pine needles possessed anti-oxidant activity in vitro.