Abstract: The β-glucosidase was immobilized on the carrier of porous ceramic balls with glutaraldehyde by crosslinking reaction. The solution composed by a certain concentration of cellubiose and glucose was continuously treated by immobilized β-glucosidase loaded on a packed bed reactor. The operation condition and stability of this immobilized enzyme during enzymatic hydrolysis were investigated. The results indicated that the optimum operation pH value and temperature were 4.8 and 60℃, respectively, in the continuous hydrolysis process. Furthermore, the conversion declined and the production efficiency on volume increased with the rise of flow rate. The conversion rate was 91.7% as the substrate flow rate and production efficiency on volume were 5.47 mL/min and 8.98 g/(L·h), respectively. The mixed cellubiose and glucose solution was continuously enzymatic hydrolyzed for 10 days under this stable flow rate at 5.47 mL/min. The conversion rate of cellubiose could be maintained at a constant lever higher than 90%, and the mean conversion rate was 90.7% in present study.