金樱子与葡萄皮中原花青素的抗氧化活性比较

A Comparative Study on Antioxidant Activities of Proanthocyanidins between Rosa laevigata and Grape Skin

摘要: 从金樱子和葡萄皮中提取原花青素并进行纯化，比较了两种纯化液对羟基自由基(•OH)、超氧阴离子自由基(O2-•)和亚硝酸盐(NO2-)体系的清除作用以及对油脂氧化体系的抑制作用。结果表明，当质量浓度为0.5g/L时，金樱子中原花青素对•OH、O2-•及NO2的清除率分别是98.32%、95.93%、96.39%，而葡萄皮中原花青素对•OH、O2-•及NO2-的清除率分别是42.21%、24.42%、38.51%，金樱子和葡萄皮中原花青素对植物油的过氧化值分别为0.098和0.103mmol/kg，表明金樱子中的原花青素抗氧化效果均强于葡萄皮中的原花青素。

Abstract: Proanthocyanidins were extracted and purified from Rosa laevigata and grape skin. The antioxidant activities of purified proanthocyanidins were investigated by testing scavening effects of ·OH, O2-·, NO2- and against the peroxidation of oils. The results showed that at the same proanthocyanidin concentration of 0.5 g/L, the scavenging rate to ·OH,O2-· and NO2- was 98.32%, 95.93%, 96.39% of proanthocyanidins from R. laevigata, and 42.21%, 24.42%, 38.51% of proanthocyanidins from grape skin, respectively. The peroxide value of oil was 0.098 and 0.103 mmol/kg for proanthocyanidins from R. laevigata and grape skin, respectively. The results indicated that purified proanthocyanidins from R. laevigata possessed better antioxidant activity than those from grape skin.