泡桐产生物乙醇稀酸预处理过程的研究

Dilute Acid Pretreatment of Paulownia for Bioethanol Production

摘要: 在121℃下进行稀酸(0.5%，1.0%，1.5%和2.0%)预处理泡桐30~90min，检测水解液中的总还原糖、各种单糖的浓度以及预处理过程中抑制物(糠醛和羟甲基糠醛)。在控制抑制物质量浓度在1g/L以下，即不影响后续酶解发酵的情况下得到良好的酶解效率。最佳预处理条件为稀酸质量分数1.5%，反应时间60min，该条件下总还原糖的得率可达到理论值的82%，葡萄糖和木糖的转化率分别达到了39.5%和48.7%。

Abstract: Dilute sulfuric acid pretreatment of paulownia before cellulose enzymatic hydrolysis was investigated. The biomass with a 10% solid loading rate was pretreated at 121℃ by dilute sulfuric acid with different concentrations (0.5%, 1.0%, 1.5% and 2.0%) for 30, 60 and 90 min. The concentrations of the total reducing sugars, monosaccharides and inhibitors (e.g. furfural and HMF(5-hydroxymethylfurfural)) in the pre-hydrolyzate were analyzed to obtain the optimum acid hydrolysis condition that did not affecting the flowing enzymatic hydrolysis. The total reducing sugar yield was about 82% of the theoretical maximum value, and the conversion efficiencies for glucan and xylan were 39.5% and 48.7%, respectively, when the biomass was pretreated by 1.5% sulfuric acid for 60 min.