Abstract: In order to find effective organic wood preservatives, the structure-activity relationship of triazolanol compounds was explored. Referencing to the chemical structure of tebuconazole, nine 1-(1H-1,2,4Vtriazol-1-yl)-2-(t-butyl)-3-substituted-2-propanol compounds were synthesized. They were characterized by MS and 1H NMR, etc. In the range of 0.5%-2%,their antimicrobial activity were evaluated by agar-well diffusion method for four kinds of wood spoilage bacteria. The expermental results showed that the para-substituted phenyl group is conducive to the antibacterial activity, and the target compounds have a good performance in anti-mildew. Moreover, the inhibitory effect of 4f against Trichoderma viride and wood-rotting fungi are considerable with tebuconazole.